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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but thorough nucleotide sequence details is not still obtainable.
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Blocking peptides are peptides that bind especially towards the goal antibody and block antibody binding. These peptide ordinarily contains the epitope identified via the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to be certain the absolute best benefits are...
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This guideline outlines additional achievable optimization of mobile and immunoassay parameters more info to guarantee the best possible outcomes are...
Antibodies bound to the blocking peptide not bind towards the epitope around the concentrate on protein. By evaluating the staining within the blocked antibody compared to the antibody on your own, one can see which staining is particular.
Target Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation patterns which lead on the temporal coordination of each mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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